BBa_K1377000
BBa_K1377000 Version 1 (Component)
promoter of pili A gene cluster + spaA front

BBa_K1796015
BBa_K1796015 Version 1 (Component)
complete line of nif cluster

BBa_K137002
BBa_K137002 Version 1 (Component)
L.lactis folate gene cluster

BBa_K302034
BBa_K302034 Version 1 (Component)
mazEF toxin-antitoxin cluster

BBa_K729000
BBa_K729000 Version 1 (Component)
Temperature regulated Gas Vesicle Polycistronic gene cluster composite

BBa_K917006
BBa_K917006 Version 1 (Component)
E. coli cytochrome maturation gene cluster ccmA-H

BBa_K1172401
BBa_K1172401 Version 1 (Component)
mtrCAB - gene cluster from S. oneidensis for cytochrome expression

BBa_K1172402
BBa_K1172402 Version 1 (Component)
mtrCAB gene cluster with IPTG inducible lac promoter and RBS

BBa_K1428001
BBa_K1428001 Version 1 (Component)
Gene cluster for Expressing a purifiable Eut Bacterial Microcompartment

Pnif
BBa_K1796001 Version 1 (Component)
promoter of Paenibacillus sp. WLY78 nif gene cluster

BBa_K917013
BBa_K917013 Version 1 (Component)
pLac promoter plus cytochrome maturation gene cluster ccmA-H

BBa_K1112002
BBa_K1112002 Version 1 (Component)
Cluster PHA

BBa_K1152002
BBa_K1152002 Version 1 (Component)
TycC5 Module from Tyrocidine cluster

rib-synth
BBa_K1172303 Version 1 (Component)
Riboflavin synthesis gene cluster from shewanella oneidensis

BBa_K1172304
BBa_K1172304 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS

BBa_K1172305
BBa_K1172305 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a medium Anderson promoter

BBa_K1172306
BBa_K1172306 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a strong Anderson promoter

BBa_K1316011
BBa_K1316011 Version 1 (Component)
pFAB640 regulating the ccmAH cluster

BBa_K302022
BBa_K302022 Version 1 (Component)
spaBTC cluster

BBa_K2088006
BBa_K2088006 Version 1 (Component)
It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I

BBa_K2088007
BBa_K2088007 Version 1 (Component)
pbaB: a 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain.

BBa_K2088692
BBa_K2088692 Version 1 (Component)
gene cluster mhbDHIM

BBa_K1951011
BBa_K1951011 Version 1 (Component)
desABCD cluster producing biosynthesis pathway to make Desferrioxamine B

406
BBa_K398406 Version 1 (Component)
Solvent tolerance cluster

bbc1
BBa_K398108 Version 1 (Component)
Salt tolerance cluster

BBa_K565001
BBa_K565001 Version 1 (Component)
Colicin G gene cluster

BBa_K565002
BBa_K565002 Version 1 (Component)
Colicin H gene cluster

mdnED
BBa_K627000 Version 1 (Component)
ABC transporter and N-acetyltransferase from mdn-cluster

mdnB
BBa_K627002 Version 1 (Component)
ATP-grasp-type ligase from mdn-cluster

mdnC
BBa_K627003 Version 1 (Component)
ATP-grasp-type ligase from the mdn-cluster

mdnD
BBa_K627004 Version 1 (Component)
N-acetyltransferase from the mdn-cluster

mdnE
BBa_K627005 Version 1 (Component)
ABC transporter from the mdn-cluster

mdnA-myc-g
BBa_K627006 Version 1 (Component)
Fusion part of mdnA gene (from mdn-cluster) with myc-tag and gene III

BBa_J72169
BBa_J72169 Version 1 (Component)
O7 biosynthesis cluster

BBa_J72170
BBa_J72170 Version 1 (Component)
K1 biosynthesis cluster

BBa_K737010
BBa_K737010 Version 1 (Component)
Gvp cluster

BBa_K737015
BBa_K737015 Version 1 (Component)
Artificial gvp gene cluster

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K195300
BBa_K195300 Version 1 (Component)
neuD,neuB,neuA,neuC,neuE(neu gene cluster).

BBa_K302017
BBa_K302017 Version 1 (Component)
spaIFEG cluster